关键词: 大鼠, 模型, Ⅲ度烫伤

Abstract: Objective　To establish an easy-to-operate and safe rat scald model with reproducible scald depth.Methods　A total of 42 healthy SPF-grade SD rats were randomly assigned into seven groups, groups A, B, C, D, E, F and control group. Rats in groups A, B and C were subjected to scald injury using a thermostatic water bath, while rats in groups D, E and F were modeled with a thermostat scald instrument, the control group underwent sham scald treatment. The ideal parameters for model creation were determined based on factors such as scald area, whether the scald depth met expectations, and survival rate of experimental animals. Additionally, serum biochemical changes induced by different scald method were assessed.Results　Rats in groups A and B exhibited second-degree scalds, while those in group C visually reached a preliminary third-degree scald level. However, the scald area did not meet expectations. Rats in groups D and E showed second-degree scald, and those in group F exhibited third degree scald with well-defined scald areas. Histological examination of skin in group F rats met the required standards. Compared with control group, the ALB levels of rats in water bath group and scald instrument group were significantly decreased. Meanwhile, the ALP and AST levels in these two groups were significantly elevated, and ALP level in the water bath group was statistically higher than that in the scald instrument group (P<0.05).Conclusion　The constant temperature and pressure scald device allows for precise control of scald depth and demonstrates excellent performance in controlling the scald area,resulting in less damage to experimental animals.

Key words: rat, model, third-degree scald